Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Mouse Neutrophil Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Neutrophil Enrichment Kit is designed to isolate neutrophils from single-cell suspensions of bone marrow or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-neutrophils. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Amsbio LLC Immunoplate for capture L1CAM positive exosomes
Immunoplate for capture L1CAM positive exosomes
Biotium Primary Antibody CD9 NA CF647Plus 1/EA
ExoBrite STORM CF647Plus CD9 (Mouse) Antibody is designed for detection of extracellular vesicles by STORM CF647Plus has been validated and recommended for STORM microscopy and has Ex/Em at 652/668 nm 500 uL
MilliporeSigma Total Protein Extraction Kit, 1 kit
Total protein extraction kit is an excellent tool for the initial purification and preparation of proteins from any tissues and cells. Total protein isolated by this kit is native and can be used for downstream applications.
AdipoGen TAPBPL msFc hrec
Recombinant Protein. The extracellular domain of mouse TAPBPL aa 21-412 is fused at the C-terminus to the Fc portion of human IgG1. Lyophilized. Contains PBS. T cells play critical roles in the adaptive immune system, protecting the human body against cancer, bacterial, viral, fungal, and parasitic infections. T cell immune response is tightly controlled by immune checkpoint proteins that negatively or positively regulate T cell response. Among the immune checkpoint proteins, the B7 family plays a key role in controlling immune responses and belongs to the immunoglobulin Ig superfamily. A number of B7 family ligands have been identified, such as B7-1 CD80, B7-2 CD86, PD-L1 B7-H1, PD-L2 B7-DC, B7-H2 inducible T cell co-stimulator ligand ICOS, B7-H3, B7-H4 B7x, B7S1, B7-H5 VISTA and B7- H6. Because of the potential clinical applications of immune checkpoint proteins, there has been intense interest in identifying additional T-cell regulators.
Amsbio LLC ExoTEST™ for Overall Exosome capture and quantification from Cell media
ExoTEST™ for Overall Exosome capture and quantification from Cell media
Invent Biotechnologies Inc Minute™ Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) - Spin Column-Based (20 Preps)
Nucleoplasm, also referred to as karyoplasm/nucleus sap, consists of chromosomes, nucleolus and nuclear matrix. Nucleoplasm plays a central role in genetic information flow and regulation of gene expression. Nuclear protein isolation has traditionally been accomplished by extraction protocol using detergent-containing buffers, which is effective in extracting total nuclear proteins but unable to separate membrane-bound proteins and water-soluble matrix proteins. The detergent-free nuclear matrix protein extraction kit is designed to separate cellular protein into three fractions: cytosolic, nuclear matrix and water-insoluble fractions (mainly envelope and nucleic acid-associated proteins) using a patented spin-column-based technology. The buffers used are detergent and EDTA-free. The proteins extracted are in their native state and can be used in many downstream applications such as gel retardation, transcription factor analysis, apoptosis and protein trafficking studies.
Invent Biotechnologies Inc Minute™ Nuclear Envelope Protein Extraction Kit - Spin Column-based (50 Preps)
The nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify because of its connection to nuclear and cytoplasmic components. Traditional methods of nuclear envelope isolation and purification require a large amount of starting material and a long, tedious procedure. Minute™ Nuclear Envelope Protein Extraction Kit is the first commercial kit designed to rapidly isolate nuclear envelope and its associated proteins in native form without using density-gradient and ultracentrifugation. Due to the use of protein extraction filter cartridges, the nuclear envelope protein isolation is simple and user-friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional methods that require large amounts of starting cells/tissues, this kit starts with only 10-20 million cells, and the buffers are detergent and EDTA free.
Invent Biotechnologies Inc Minute™ Adipose Tissue Fractionation Kit - Spin Column-Based (20 Preps)
On average, total cellular proteins account for less than 2% of adipose tissue. Adipose tissue, especially white adipose tissue, has been recognized as an important endocrine and inflammation organ in addition to its energy storage function. Fractionation and analysis of proteins from adipose tissue are critical for understanding many physiological/pathological conditions. However, fractionation of adipose tissue is challenging due to its high lipid and low protein contents. The water-oil emulsion present in biological samples is notoriously difficult to separate, but we have developed a cutting-edge technology to deal with this issue: a porous filter with unique surface property and predefined pore size coupling that has a specially formulated detergent-free fractionation buffer.
Genscript Corporation CytoSinct CD4 Nanobeads huma
CytoSinct CD4 Nanobeads human is used for in vitro enrichment of CD4 cells from fresh or frozen peripheral blood mononuclear cells (PBMCs) leukapheresis products or single-cell suspension based on the surface expression of human CD4
Mayflower Bioscience TRIFic CD9 Exosome Assay, 96 well
The TRIFic™ exosome detection assay delivers clear and consistent data quickly by providing quantitative data from purified or unpurified samples, including direct measurement of exosomes in plasma in a convenient 96-well format. It is similar to an ELISA assay, but the target is detected directly with a Europium label without any enzymatic reaction. TRIFic™ exosome detection assays are being made available for widely used exosome markers, allowing for identification of CD9+/CD81+/CD63+ - EVs.
Genscript Corporation Her2/ErbB2 His Mouse
ErbB2 also called Neu and Her2 (human epidermal growth factor receptor 2) is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors Upon ERBB2 activation the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane This prevents the phosphorylation of APC and CLASP2 allowing its association with the cell membrane
Mayflower Bioscience TRIFic CD63 Exosome Assay, 96 well
The TRIFic™ exosome detection assay delivers clear and consistent data quickly by providing quantitative data from purified or unpurified samples, including direct measurement of exosomes in plasma in a convenient 96-well format. It is similar to an ELISA assay, but the target is detected directly with a Europium label without any enzymatic reaction. TRIFic™ exosome detection assays are being made available for widely used exosome markers, allowing for identification of CD9+/CD81+/CD63+ - EVs.
Tosoh Bioscience LLC TSKGEL AMIDE-80 2X250 5UM
Column, Normal Phase/Hydrophilic Interaction Chromatography; Tosoh TSKgel Amide-80; Media: Silica; Particle: 5 micrometer; Pore Size: 80 angstrom; 2mm I.D. x 25cm Length; Stainless Steel